简介:4-硝基苯基-N-乙酰-β-D-氨基葡糖苷用于快速比色测定人尿中 N-乙酰基-β-氨基葡萄糖苷酶的有用底物。
4-硝基苯基-N-乙酰-β-D-氨基葡糖苷物理化学性质:
密度
1.5±0.1 g/cm3
沸点
694.7±55.0 °C at 760 mmHg
熔点
210 - 212oC
分子式
C14H18N2O8
分子量
342.301
闪点
374.0±31.5 °C
精确质量
342.106323
PSA
154.07
LogP
0
外观性状
白色粉末
蒸汽压
0.0±2.3 mmHg at 25°C
折射率
1.62
储存条件
常温,避光,通风干燥处,密封保存
稳定性
常温常压下稳定
水溶解性
H2O: 5 mg/mL, clear, colorless to very faintly yellow
4-硝基苯基-N-乙酰-β-D-氨基葡糖苷详细介绍:
中文名称:
4-硝基苯基-N-乙酰-β-D-氨基葡糖苷
英文名称:
N-[(2s,3r,4r,5s,6r)-4,5-Dihydroxy-6-(Hydroxymethyl)-2-(4-Nitrophenoxy)oxan-3-Yl]ethanamide
CAS号:
3459-18-5
详细描述:
创赛优选商城提供的[Sigma-Aldrich]4-Nitrophenyl N-Acetyl-β-D-Glucosaminide为创赛科技出品,产品稳定,定制速度快,极大地方便了广大科研客户的使用需求。产品包装为500MG,安全运输。
属性:
质量水平:300
测定:≥99% (TLC)
形式:powder
杂质:≤0.05% Free p-nitrophenol
溶解性:water: 5 mg/mL, clear, colorless to very faintly yellow
储存温度:?20°C
SMILES string:CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
InChI:1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
InChI key:OMRLTNCLYHKQCK-DHGKCCLASA-N
应用:
N-acetyl-β-D-glucosaminidase (NAG) has been used:
as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)
as a substrate to study chitinase ChiB1 activity
to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells
包装:
100, 250, 500 mg in poly bottle
1 g in poly bottle
生化/生理作用:
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
底物:
N-乙酰-氨基葡糖苷酶的发色底物。