产品名称：Recombinant Human Glial Cell Line-derived Neurotrophic Factor（rHu GDNF）

产品价格：

产品介绍：

Synonyms	ATF, ATF-1
Accession	P39905
GeneID	2668
Source	Escherichia coli.
Molecular Weight	Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Quantity	2μg/10μg/1000μg
AA Sequence	SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 × 107 IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, with 0.05 % Tween-20.
Endotoxin	Less than 0.1 EU/μg of rHuGDNF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage	This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
SDS-PAGE
Reference	1. Wood MD, Kim H, Bilbily A, et al. 2012. Muscle Nerve, 46: 122-4. 2. Stahl K, Mylonakou MN, Skare O, et al. 2011. Brain Res, 1378: 105-18. 3. Cornejo M, Nambi D, Walheim C, et al. 2010. Neurochem Res, 35: 1643-51. 4. Boado RJ, Zhang Y, Wang Y, et al. 2008. Biotechnol Bioeng, 100: 387-96.
Background	Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Rat and human mature GDNF shows 93 % sequence identity and have species cross-reactivity.

Primegene重组蛋白六大优势：

★高纯度、高蛋白活性

★低内毒素

★不同批次 产品稳定

★国内现货、高效快速

★Bio-Techne旗下品牌，品质保障

★无缝替代进口蛋白，质优价廉。

Primegene蛋白分装小贴士：

Primegene（普欣）是美国Bio-Techne（原R&D）旗下子品牌，专注于重组蛋白研发、生产， 产品涵盖分子生物学、蛋白化学、细胞生物学、免疫学等生命科学的诸多领域，拥有蛋白表达，菌种发酵，蛋白纯化，蛋白冻干以及抗体制备等完整的技术平台，并拥有自己的GMP中试生产车间。重组蛋白产品500多个，覆盖20余个物种及多项专利技术。

上海怡淼化学科技有限公司，作为美国Bio-Techne（原R&D）旗下子品牌Primegene（普欣）授权代理商，为抗肿瘤药物、细胞治疗、细胞培养、美容产品等领域的客户提供优质的产品和服务，同时可以提供：Perfemiker（博飞美科）、Sigma（西格玛）、Gibco、Jackson、TCI（梯希爱）、Alfa（阿法埃莎）、Acros、Thermo Fisher（赛默飞）等多种品牌，为您的科研提供一站式解决方案，欢迎咨询。

更多产品信息，请联系客服咨询。