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产地 | 中国 |
品牌 | PrimeGene(普欣) |
货号 | 101-03 |
用途 | 细胞/科研/抗肿瘤药物 |
包装规格 | 2μg |
纯度 | > 96 %% |
CAS编号 | |
是否进口 | 否 |
产品名称:Recombinant Human Interleukin-3(rHuIL-3)
产品价格:
货号 | 规格 | 价格 |
101-03 | 2μg | 600 |
101-03 | 10μg | 1500 |
101-03 | 100μg | 5510 |
101-03 | 250μg | 9920 |
101-03 | 500μg | 12900 |
101-03 | 1mg | 17640 |
产品介绍:
Synonyms | Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor. |
Accession | P08700 |
GeneID | 3562 |
Source | Escherichia coli. |
Molecular Weight | Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
Quantity | 2μg/10μg/1000μg |
AA Sequence | APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF |
Purity | > 96 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin | Less than 1 EU/μg of rHuIL-3 as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Usage | This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE | ![]() |
Reference | 1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10. 2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95. 3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24. 4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41. |
Background | Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells. |
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