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产品描述

General description

DIG DNA Labeling Mix is an easy-to-use labeling mixture for rapid random-primed labeling with digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP). DIG-dUTP is incorporated every 20 to 25 nucleotides in the newly synthesized DNA. This density of haptens in the DNA yields the highest sensitivity in the detection reaction.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

The DIG DNA labeling mix has been used in a variety of hybridization techniques including:
 Southern blots^[2]
northern blots
 dot blots
screening of gene libraries
in situ hybridizations^[3]

Features and Benefits

内容
10x 浓缩dNTP标记混合物：1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM DIG-dUTP, 碱性不稳定, pH 7.5(+20℃)

分析时间：标记：1小时O/N
标记效率：每次实验使用1μgDNA，1小时后，大约10%核苷酸插入大约250ng新合成标记DNA；20小时后，大约30%核苷酸插入大约750ngDNA。

Quality

DIG DNA标记试剂盒和DIG核酸检测试剂盒功能测试。

Principle

根据随机引物标记方法，生产DIG-标记DNA探针。这种方法是随机寡核苷酸和变性DNA模板杂交。互补DNA链合成使用Klenow酶，3′-OH末端随机寡核苷酸作为引物，以及含有DIG-11-DUTP，碱性不稳定的脱氧核糖核苷酸混合物进行延长反应。在新合成的DNA中，每隔20到25个核苷酸插入DIG-DUTP。DNA中半抗原密度使得检测反应灵敏度*。

Preparation Note

样本材料
作为标记反应的模板

至少100bp的DNA片段
线性质粒，柯斯质粒或λDNA
超螺旋 DNA
或最少（10ng）DNA量比如，从低融点凝胶中分离出来的DNA限制性片段也可以用。

注意：线性DNA比起环状和超螺旋DNA标记效率更高。

Other Notes

仅用于生命科学研究。不可用于诊断。