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产品描述

General description

DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Luminescent Detection Kit has been used in:
critical commercial assays^[3]
Southern hybridization^{[1] [4]}
northern analysis^[2]
plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD^{[1] [2]}

包装

1个试剂盒包含5种组分。

Specifications

灵敏度：在<30分钟的暴露时间后，在Southern或斑点印迹中可检测到0.03pg的同源DNA或0.1pg的同源RNA。在0.3 μg哺乳动物基因组DNA中可检测到单拷贝基因。

Preparation Note

工作浓度：0.5mg/ml，用于流式细胞术
储存条件（工作溶液）：抗体结合物（瓶3）：一旦打开，应储存在2至8℃
封闭试剂（瓶4）：溶液状态，应储存在在2-8℃
CSPD（瓶5）：频繁使用时在储存在2至8℃。应避免反复冻/融循环。

碱性磷酸酶对二氧杂环丁烷CSPD的酶促去磷酸化会生成亚稳态酚盐阴离子，其会在477nm处分解并发光。发光会随时间增加，直到达到恒定的强度。
检测时间
免疫检测1.5小时
信号检测5至30分钟

注意：避光存放。

Other Notes

仅用于生命科学研究。不可用于诊断。