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产品描述

General description

The DIG Northern Starter Kit is designed for the novice DIG system user. It contains the reagents proven to provide successful, reproducible results in northern blots. Additionally, the more convenient forms of standard DIG system products are included (e.g., CDP Star, ready-to-use). The small number of reactions allows the user to gain a firm foundation using the DIG system, then "graduate" to the standard pack sizes. For optimum success, use this kit with the DIG Wash and Block Buffer Set.This kit is used for the generation of single-stranded, DIG-labeled RNA probes and chemiluminescent detection. Labeled probes are synthesized by the in vitro transcription method.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Northern Starter Kit可制备与随附的化学发光检测试剂联用进行RNA印迹检测的DIG标记RNA探针。以线性化DNA为模板，利用SP6、T3或T7 RNA聚合酶将DIG-11-UTP整合到RNA转录物中。标记后，DIG-标记的探针即可用于杂交。为了方便起见，DIG Easy Hyb可用于杂交。直接向瓶中加入64 ml的DEPC处理（无RNase）水，即可轻松复溶DIG Easy Hyb（复溶的DIG Easy Hyb可在室温下稳定储存1个月）。杂交完成后,杂交液中包含DIG-标记的RNA探针，可保存于-15到-25°C，以备后续使用（最多可保存1年）。

DIG Northern Starter Kit已用于杂交分析乙型肝炎病毒(HBV) RNA、2型柑橘麻风病毒（CiLV-C2）RNA。^{[1] [2]}

包装

1个试剂盒含11种组份。
1个试剂盒至多可进行10次标记反应和10次印迹检测，印迹膜大小为10×10cm²，μg DNA反应物，各获得约20μg标记RNA

灵敏度和特异性：采用1μg线性DNA模板，标记反应一般会在1小时内得到20μg新合成的DIG标记RNA。DIG-标记的RNA探针可以在斑点印迹中检测到0.1pg的同源DNA或RNA。可在0.1μg总RNA中检测到*的mRNA。

Preparation Note

工作溶液：注：使用无菌、无RNAase的溶液和仪器
分析时间：9小时30分钟
样本材料：
线性质粒，含适当的RNA聚合酶启动子序列（SP6,T3,T7）。
专为PCR产物制备
工作溶液：注：使用无菌、无RNAase的溶液和仪器。

注：待转录区域的长度应在200到1000bp之间。为避免RNase污染，DNA必须酚化处理。

Other Notes

仅用于生命科学研究。不可用于诊断。