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产品描述

General description

Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.

Application

Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH)^[5]  and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Features and Benefits

*通过耐碱性醚键与脱氧尿苷三磷酸结合。

耐碱性：在+ 15至+ 25℃条件下，该制剂在0.1-0.5M NaOH 存在时稳定。

Quality

典型分析：至少 85％ DIG-11-dUTP（HPLC，面积％）。
通过 RPL 测试的功能。

Preparation Note

工作溶液：我们建议制备含有dATP、dCTP、dGTP、dTTP（各 10mM）的脱氧核苷酸混合物；（例如，为了制备100μl 核苷酸混合物，将 10μl dATP、dCTP、dGTP、dTTP（各）加至 60μl 水，PCR 级）。
储存条件（工作溶液）：在15至25℃条件下，4周内可能发生约 5％的分解。

Other Notes

仅用于生命科学研究。不可用于诊断。