即日起至9月30日，精选Roche畅销产品大促!

产品描述

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Specificity

来自绵羊的多克隆抗体对*配基和*具有特异性，并且与其他类固醇（如人类雌激素和雄激素）没有交叉反应性。

Application

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
whole-mount in situ hybridization
fluorescence in situ hybridization
enzyme linked immunosorbent assay (ELISA)
dot blot
Southern blot
western blot
double labeling experiments
lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Preparation Note

工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：? 斑点印迹：50至100mU/ml
ELISA：2至50mU/ml
蛋白质印迹：50至100mU/ml
Southern印迹：50至100mU/ml

工作溶液: 100mM Tris-HCl，150mM NaCl，pH 7.5。
1％封闭试剂(w/v)，1至5％热灭活的胎牛血清(v/v)或绵羊正常血清（可用于减少非特异性结合）。

Reconstitution

加入1ml双蒸水至终浓度为50U/ml。

Other Notes

仅用于生命科学研究。不可用于诊断。