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产品描述

General description

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

Application

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.

Features and Benefits

Ease access to difficult templates:-
including GC-rich targets and repetitive sequences.
Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
Amplify DNA fragments up to 5 kb.
Cost-effective:-
use the convenient premixed solution of PCR grade NTPs.

包装

1 kit containing 6 components

Quality

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.

Unit Definition

Volume Activity: 2 U/μl

Preparation Note

Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

Other Notes

仅用于生命科学研究。不可用于诊断。