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产品描述

General description

The Titan One Tube RT-PCR Kit is designed for fast, sensitive and reproducible analysis of RNA by high fidelity reverse transcription polymerase chain reaction (RT-PCR) in a one-step reaction.

Titan^™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand^™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.

Specificity

反应特异性很大程度上取决于引物设计和DNA污染情况。
为了排除DNA靶向检测中的人为干扰，反应中应引入适当的阳性对照与阴性对照。
热灭活：94℃下处理2分钟
逆转录酶AMV

Application

Titan One Tube RT-PCR Kit has been used:
to complete cDNA synthesis in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
to amplify full-length and truncated cDNA fragments of C-X-C motif chemokine 5 (CXCL5)

Features and Benefits

• 基于所携带的校正能力，降低PCR反应过程中的出错率

  。保真性保真性超出Taq DNA聚合酶三倍之多。

• 以三种高效酶蛋白来提供更高的灵敏度。
  比传统的两步法RT-PCR更快。

  
  

  包装

  1个试剂盒包含9个组分。

  Other Notes

  仅用于生命科学研究。不可用于诊断。