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产品描述

General description

The Titan^™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand^™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
Delivers increased sensitivity due to the high efficiency of all three enzymes.
Is faster than conventional two-step RT-PCR.

Specificity

反应特异性很大程度上取决于引物设计和DNA污染情况。
为了排除DNA靶向检测中的人为干扰，反应中应引入适当的阳性对照与阴性对照。
热灭活：94℃下处理2分钟
逆转录酶AMV

Application

Titan^™ One Tube RT-PCR System has been used:
to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA^{[2] [1]}
for determining the presence or absence of RNA templates
in cloning of rare messages without the need to construct cDNA libraries
for quantifying the levels of gene expression

Features and Benefits

Titan^™ One Tube RT-PCR System:
High Fidelity: Expand^™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
offers a threefold higher fidelity in comparison to Taq DNA polymerase
delivers increased sensitivity due to the high efficiency of all three enzymes
faster than conventional two-step reverse transcriptase (RT)-PCR
allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand^™ PCR System

包装

1 套包含 4 种成分的试剂盒

Other Notes

仅用于生命科学研究。不可用于诊断。