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产品描述

General description

This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)₁₅ primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.

Application

Primer for cDNA synthesis may be used in:
RT-PCR
Generation of cDNA libraries
Synthesis of first-strand cDNA

Unit Definition

8 nmol ξ 1 A₂₆₀ unit

Other Notes

仅用于生命科学研究。不可用于诊断。