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产品描述

General description

cOmplete ULTRA 片剂含有*大的蛋白酶抑制剂混合物，可有效防止您的蛋白质被蛋白酶降解。由于这种片剂里含有EDTA，金属蛋白酶也会被抑制。本品为片剂，采用EASYpack吸塑包装。EASYpack提供了非常便利的传递方式 - 易于将单片从吸塑箔泡中直接推到缓冲液中。一片cOmplete ULTRA Mini片剂可用于10ml提取溶液。
重组蛋白在表达过程中可能有部分降解。可溶性降解产物经过共纯化，可产生具有不同长度蛋白质片段的非均质蛋白质制剂。金属蛋白质受EDTA抑制，在细菌等生物中非常普遍。

在10ml缓冲液中加入一片cOmplete ULTRA Mini蛋白酶抑制混合物和一片PhosSTOP磷酸酶抑制混合物，就可以方便地同时抑制蛋白酶和磷酸酶。

Application

During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche ′s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).

cOmplete^™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay buffer (RIPA buffer).^{[1] [2]}  It has also been used to prevent protein degradation and coagulation in blood samples.^[3]

Features and Benefits

Ultra efficient protection
The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.
Convenient tablet format
Eliminate weighing small amounts, and instantly protect your proteins against an exceptionally wide range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.
Suitable for IMAC purification
Purify poly-histidine tagged proteins.
Ensure laboratory safety
Avoid contact with hazardous compounds using cOmplete ULTRA Tablets.

Physical form

箔泡包装片剂。一片足够10 ml提取溶液使用。

Preparation Note

工作浓度：每10 ml提取液加入1片。
工作溶液：处理

使用拇指底（不是指甲）小心地将片剂推出箔包装，以防止片剂破裂。

工作溶液的制备

一片足够抑制10ml提取溶液中的蛋白水解活性。当蛋白水解活性非常高时，应将2-3片用于10 ml提取缓冲液中。片剂可以直接加入提取培养基。

储备溶液（2X浓度）

除了直接用于裂解液，也可以按照以下步骤，制备2X储存液
#8226PE或PP管中加入5ml双蒸馏水，加入cOmplete ULTEA片剂
涡旋震荡3分钟，或者直到片剂完全溶解即可。
即使储存液中有明显的小颗-粒，也是可以立即使用的。这些小颗粒不会影响片剂的抑制表面
2到8℃，储备溶液能顾保存4周；如果想要存放4周以上，请保存在  -15 to  -25℃。
2X储备溶液保存过程中可能发生小颗粒沉淀。使用前，短暂涡旋储备溶液。

储存条件（工作溶液）：溶剂为水的储备溶液可在2至8°C下稳定保存4周，在-15至-25℃下保存至少4周。

Reconstitution

溶于水性缓冲液，或直接添加到提取溶液中。

储备溶液仅能使用水。

Other Notes

仅用于生命科学研究。不可用于诊断。