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产品描述

General description

Poly（A）被用作DNA和RNA定量沉淀的载体。

Application

Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.

Poly（A）已可用于微滴式数字PCR（ddPCR）分析。

Biochem/physiol Actions

Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.

Quality

典型分析：
2.3μmol/mg Poly(A)（来自吸光度）相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A₂₅₀/A₂₆₀、A₂₈₀/A₂₆₀、A₂₉₀/A₂₆₀

Sequence

链长为 2.100 至 10.000 个核苷酸

Unit Definition

1个A₂₆₀ 单位相当于40 μg ssDNA。

Physical form

冻干品，钾盐

Preparation Note

工作浓度：0.5 mg/ml
建议浓度为0.5 mg/ml。
工作溶液：建议浓度为0.5 mg/ml。

Other Notes

仅用于生命科学研究。不可用于诊断。