纯度

> 95 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 μg/ml (100 μl/well) can bind mouse CD117 with a linear range of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/mL.

蛋白构建

A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus.

Uniprot No.

P20826-1

表达宿主

Baculovirus-Insect Cells

种属

Mouse

预测 N 端

Lys 26

分子量

The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Please contact us  for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.